BALB/c Farelerinde Oluşan Oksidatif Stres Üzerine Gümüş Nanopartiküllerle İndüklenmiş Penisilaminin Koruyucu Etkisinin Değerlendirilmesi

Fatemeh NAMAZI, Mehdi FAZELI, Yalda ZOLGHADRI, Saeed MOHAMMADINEZHAD, Saeed NAZIFI
501 130

Öz


Gümüş nanopartiküllerinin olumsuz etkilerine rağmen uygulamaları hızla artmaktadır. Bu çalışma nanogümüş uygulamasının histopatolojik değişiklikler ve biyokimyasal parametreler üzerindeki istenmeyen etkilerini ve aynı zamanda penisilaminin terapötik etkinliğini araştırmak için düzenlenmiştir.  Altışarlı dört grup BALB/c faresine değişik dozlardaki nanogümüş intraperitoneal yoldan uygulandı ve LD50 hesaplandı. Penisilaminin terapötik etkinliğini araştırmak amacıyla 42 fare eşit yedi gruba ayrıldı. Her gruptaki hayvanlara LD50 nanogümüş ve eşzamanlı olarak ya da nanogümüş enjeksiyonundan 4 saat sonra 50 ya da 200 mg/kg penisilinamin  intraperitoneal yoldan uygulandı. Toplam protein, albumin, toplam globulin, kolesterol, trigliserit, aspartat aminotransferaz (ALT), alanin aminotransferaz (ALT), alkalin fosfataz (ALP), toplam antioksidan kapasite (TAC) ve malondialdehit (MDA) de dahil olmak üzere serum biyokimyasal ve histopatolojik analizler yürütüldü. LD50 1500 ppm’di. Sonuçlar nanogümüş alan farelerde AST ve ALT enzimleri ve MDA düzeylerinde artış gösterirken TAC düzeylerinde azalma oluştu. Bu farklılıklar özellikle nanogümüş alan fareler ile nanogümüş ve 200 mg/kg penisilamin alan farelerde istatistiksel olarak anlamlıydı. Ayrıca, 1500 ppm nanogümüş alan farelerin doku bölümlerinin histopatolojik incelenmesinde karaciğer ve böbrek hasarı olduğu görüldü. Öte yandan, nanogümüşe maruz kalmış farelere karşılık 200 mg/kg penisilamin alan farelerde biyokimyasal parametreler ve doku lezyonlarında iyileşme oldu ve oksidatif stres boyutunun azaldığı belirlendi.


Anahtar kelimeler


Gümüş nanopartikülleri, oksidatif stres

Tam metin:

PDF (English)


DOI: http://dx.doi.org/10.16988/iuvfd.2015.93581

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