Evaluation of Some Antioxidant Enzymes in Cattle Infected with Foot and Mouth Virus

Ameneh KHOSHVAGHTI, Atbin ASKARI, Saeed NAZIFI, Mohsen GHANE
1.041 335

Öz


ABSTRACT

The present study was conducted to evaluate the activities of superoxide dismutase, glutathione peroxidase, malondialdehyde, catalase and their correlation in cattle infected with foot and mouth virus (FMD). Forty cows were diagnosed and confirmed for FMD. Ten clinically healthy adult cattle were selected as control. Superoxide dismutase (SOD) glutathione peroxidase (GPx), malondialdehyde (MDA) and catalase (CAT) were measured by validated standard methods. SOD and GPx were significantly lower in the infected animals compared with the healthy group (P<0.05 in all cases). The mean concentration of malondialdehyde (MDA) was significantly higher in infected animals compared with healthy cows (P<0.05). There were no significant differences between the mean activities of catalase in control group compared with infected group. There were significant associations between (GPx) and catalase (CAT) in the control group. In the FMD group, significant associations were observed for MDA with catalase. The results of this study revealed that SOD, GPx decreased and MDA increased in FMD-infected cattle in response to viral infection. However, in the future more detailed future studies are required to characterize such responses and to improve the development of novel control strategies against FMD.

Key Words: Superoxide dismutase, glutathione peroxidase, malondialdehyde, catalase, Foot and mouth disease

 

 

ÖZET

ŞAP VİRÜSÜ İLE İNFEKTE SIĞIRLARDA BAZI ANTİOKSİDAN ENZİMLERİN DEĞERLENDİRİLMESİ

Bu çalışma şap virüsü (FMD) ile infekte sığırlarda süperoksit dismutaz, glutatyon peroksidaz, malondialdehit, katalaz aktivitelerini ve bunların bağıntılarını değerlendirmek için yapılmıştır. Kırk adet ineğin FMD yönünden tanısı ve konfirmasyonu yapıldı. Kontol grubu olarak on tane klinik olarak sağlıklı yetişkin inek belirlenmiştir. Süperoksit dismutaz (SOD), glutatyon peroksidaz (GPx), malondialdehit (MDA) ve katalaz (CAT) düzeyleri geçerli standart yöntemler ile ölçüldü. İnfekte hayvanlarda SOD ve GPx değerleri kontrol grubu ile karşılaştırıldığında (P<0,05 her vakada) anlamlı olarak düşük bulundu. İnfekte hayvanlarda malondialdehit (MDA)'in ortalama konsantrasyonu kontrol grubu ile karşılaştırıldığında (P<0,05) anlamlı olarak daha yüksek bulundu. İnfekte grubu ile kontrol grubu karşılaştırıldığında katalaz düzeylerinin ortalama değerleri arasında anlamlı bir değişiklik yoktu. Kontrol grubu içinde GPx ve katalaz (CAT) değerleri arasında anlamlı bir ilişki saptandı. İnfekte grubunda katalaz ile MDA değerleri arasında anlamlı bir ilişki gözlemlendi. Bu çalışmanın sonucunda, şap virüsü ile infekte sığırlarda viral infeksiyona yanıt olarak SOD ve GPx değerlerinin azaldığı ve MDA değerlerinin arttığı belirlenmiştir. Ancak, gelecekte daha detaylı çalışmalar sayesinde bu gibi antioksidan enzimleri değişimlerinin karakterize edilmesi ve şap hastalığına karşı yeni kontrol stratejilerinin geliştirilmesi mümkün olacaktır.

Anahtar Kelimeler: Süperoksit dismutaz, glutatyon peroksidaz, malondialdehit, katalaz, Şap hastalığı

Anahtar kelimeler


Süperoksit dismutaz, glutatyon peroksidaz, malondialdehit, katalaz, Şap hastalığı

Tam metin:

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DOI: http://dx.doi.org/10.16988/iuvfd.98796

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