Anti-Inflammatory Effects of Insulin and Dexamethasone on Experimentally Escherichia coli Serotype O55:B5 Induced Endotoxemia in Iranian Fat-Tailed Sheep

Aliasghar CHALMEH, Khalil BADIEI, Mehrdad POURJAFAR, Saeed NAZIFI
761 367

Öz


ABSTRACT

The present experiment was performed in order to compare the effect of insulin and dexamethasone on treating endotoxemia following the intravenous infusion of endotoxin of Escherichia coli serotype O55:B5 in the Iranian fat-tailed sheep. Twenty clinically healthy one-year old Iranian fat-tailed ewes were randomly assigned into four equal (n=5) experimental groups, comprising of Control, Dexa, Insln 1.5 and Insln 3 groups. Lipopolysaccharide from Escherichia coli serotype O55:B5 was administered intravenously at 20 µg/kg. All experimental groups undergone intravenous fluid therapy for 120 minutes after lipopolysaccharide injection. 180 minutes after lipopolysaccharide injection, dexamethasone (in Dexa group at 1 mg/kg) and insulin regular (in Insln 1.5 and 3 at 1.5 and 3 IU/kg, respectively) were injected along with the intravenous fluid for 60 minutes. The Control group just received lipopolysaccharide and was treated only with intravenous fluid without using any drug. The blood samples were collected from all ewes and assayed separated sera for serum acute phase proteins (serum amyloid A and haptoglobin), inflammatory cytokines (tumor necrosis factor-alpha and interferon-gamma) and oxidative stress biomarkers (super oxide dismutase and glutathione peroxidase). In all experimental groups a rapid increase was seen in the amount of acute phase proteins and inflammatory cytokines and a decrease was seen in oxidative stress biomarkers after endotoxemia induction (P<0.05). The results of the present study showed that insulin regular at 3 IU/kg controlled acute phase response following endotoxemia induction. The potency of insulin regular at 3 IU/kg was significantly higher than 1.5 IU/kg and dexamethasone at 1 mg/kg (P<0.05) in order to treat endotoxemia due to Escherichia coli serotype O55:B5 in Iranian fat-tailed sheep.

Keywords: Endotoxemia, treatment, insulin regular, dexamethasone, Iranian fat-tailed sheep

 

ÖZET

YAĞLI KUYRUKLU İRAN KOYUNLARINDA DENEYSEL ESCHERICHIA COLI SEROTİP O55:B5'E BAĞLI ENDOTOKSEMİDE İNSÜLİN VE DEKSAMETAZONUN ANTİ İNFLAMATUAR ETKİLERİ

 

Bu deneyde yağlı kuyruklu İran koyunlarında Escherichia coli serotip O55:B5'e ait endotoksinlerin intravenöz infüzyonunu takiben insulin ve deksametazonun endotoksemi tedavisindeki etkileri karşılaştırıldı. Yirmi adet klinik olarak sağlıklı bir yaşındaki yağlı kuyruklu İran koyunların tesadüfi seçimiyle Kontrol, Dexa, Insln 1,5 ve Insln 3'ten oluşan dört (n=5) adet deney grubu oluşturuldu. Escherichia coli serotip O55:B5'in lipopolisakkaridi 20 µg/kg dozda intravenöz olarak uygulandı. Tüm deney gruplarına lipopolisakkarit enjeksiyonu sonrası 120 dakika süreyle intravenöz sıvı tedavisi uygulanmıştır. Lipopolisakkarid enjeksiyonundan 180 dakika sonra, deksametazon (Dexa grubuna 1 mg / kg) ve insülin (Insln 1,5 ve 3 gruplarına sırasıyla, 1,5 ve 3 IU / kg) 60 dakika süreyle damar içi sıvı ile birlikte enjekte edildi. Kontrol grubuna sadece lipopolisakkaritten oluşan ve herhangi bir ilacın kullanılmadığı intravenöz sıvı tedavi  uygulandı.Tüm koyunlardan kan örnekleri toplanarak elde edilen serumlardan akut faz proteinleri (serum amiloid A ve haptoglobin), inflamatuar sitokinler (tümör nekroz faktörü-alfa ve interferon-gamma) ve oksidatif stres belirteçleri (süper oksit dismutaz ve glutatyon peroksidaz) değerleri ölçülmüştür. Bütün deney grupları içinde endotoksemi indüksiyondan sonra (P<0,05), akut faz proteinleri ve inflamatuar sitokin miktarlarında hızlı bir artış görülürken oksidatif stres belirteçlerinde düşüş görülmüştür. Bu çalışmanın sonucunda endotoksemi indüksiyonundan sonra 3 IU / kg dozda insulinin akut faz yanıtını  kontrol ettiği görülmüştür. Yağlı kuyruklu İran koyunlarda Escherichia coli serotip O55:B5'e bağlı endotokseminin tedavisinde 3 IU/kg insülinin etkisi 1,5 IU/kg doz insülinden ve 1 mg/kg deksametazonun etkisinden anlamlı derecede yüksek olduğu (P<0,05) belirlenmiştir.

Anahtar Kelimeler: Endotoksemi, tedavi, insulin, deksametazon, yağlı kuyruklu İran koyunu


Anahtar kelimeler


Endotoksemi, tedavi, insulin, deksametazon, yağlı kuyruklu İran koyunu

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