Erken evre meme kanserinde revers transkriptaz polimeraz zincir reaksiyonu (RT-PCR) tekniği ile kemik iliği mikrometastazlarının araştırılması

Neslihan CABIOĞLU, Engin Okan YILDIRIM, Özlem BİTİŞİK, Hülya YAZICI, Mahmut MÜSLÜMANOĞLU, Nejat DALAY, Abdullah İĞCİ, Vahit ÖZMEN, Temel DAĞOĞLU, Mustafa KEÇER
518 184

Öz


AMAÇ Meme kanserinde kemik iliği mikrometastazı saptanmasında sitokeratin 19 (CK19) ve DF3 gen ekspresyonlarının araştırıldığı moleküler bir yöntemin etkinliğini değerlendirmektir. GEREÇ VE YÖNTEM Klinik olarak aksilla negatif olan 28 erken evre meme kanserli hastadan alınan kemik iliği aspiratlarında revers transkriptaz polimeraz zincir reaksiyonu (RT-PCR) tekniğiyle CK19 ve DF3 genlerine özgü iki ayrı primer kullanılarak mikrometastatik kanser hücresi araştırıldı. BULGULAR Hastaların ortanca yaşı 47,5'tir (32-86). RT-PCR çalışmalarında negatif kontrol olarak kullanılan kronik miyeloid lösemili kemik iliği örneklerinin keratin 19 ekspresyonun pozitif ve DF3 ekspresyonunun ise negatif bulunması üzerine, sonraki deneylerde DF3 primeri kullanı ldı. Yirmi sekiz hastanın 18'inde (%64) DF3 pozitif olarak saptandı. DF3 pozitifliği ile hastaların evresi, tümör büyüklüğü (< 2 cm ve > 2 cm), aksiller tutulum, ER/PR pozitifliği ve HG veya NG yüksekliği gibi parametreler arasında istatistiksel açıdan anlamlı bir fark bulunamadı. SONUÇ Erken evre meme kanserli hastalarda kemik iliğinde RT-PCR ile DF3 gen ekspresyonunun varlığının araştırılması yoluyla mikrometastaz saptanması tekniğinin, spesifikliğinin ve duyarlılığının yüksek olması nedeniyle etkin bir yöntem olduğu düşünülmektedir.

Anahtar kelimeler


Meme neoplazmları,Ters transkriptaz polimeraz zincir reaksiyonu,Kemik iliği neoplazmları,Kemik iliği,Neoplazm metastazı,Keratin-19,Gen ekspresyonu,DF3 meme-karsinom-ilişkili antijen

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Referanslar


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