Dehalogenation and detoxification of 2,4-dichlorophenol with induced laccase

Eda Benli, Serap Gedikli, Pınar Aytar, Gökhan Güngörmedi, Ahmet Çabuk
1.133 508


2,4-dichlorophenol is a recalcitrant compound which is used in the production of pesticides. Chlorine removal of 2,4-dichlorophenol with the crude laccase produced Trametes versicolor ATCC (200801) in potato dextrose broth including wheat bran was investigated. The optimization parameters for dechlorination such as pH, initial substrate concentration, reaction period, reaction temperature and amount of enzyme were examined. At the end of these studies, pH 4, 400 μM of initial substrate concentration, 7 min of reaction time, 30 ºC of temperature were selected. It was tested that correlation with oxygen consumption and dechlorination processes under the determined optimum conditions before and the decrease of dissolved oxygen was observed. Also, after the dechlorination of this compound, changes in chemical structure of the compound were determined with FTIR analysis and toxicity alterations with Microtox test were studied. Wide and intense peaks at spectra may be an evidence to dechlorination occurring with load transfers and electrostatic interactions. Toxicity experiments showed that 2,4-DCP was detoxified in addition to dechlorination. Besides, statistical analyses were performed for dechlorination experiments.
Keywords: 2,4-DCP, laccase, dechlorination

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