NİTRİK OKSİT SENTAZ 3 (NOS3) GLU298ASP GEN VARYASYONUNUN MEME KANSERLİ HASTALARDA İNCELENMESİ

CANAN CACINA, SOYKAN ARIKAN, YEMLİHA YILDIZ, BAHAR TOPTAŞ, SAİME TURAN, NAZLI EZGİ ÖZKAN, GURBET KORKMAZ, SEDEN KÜÇÜCÜK, ILHAN YAYLIM, TURGAY İSBİR
502 409

Öz


      Nitrik Oksit'in (NO) tümör biyolojisindeki rolü tam olarak anlaşılamamış olmasına karşın  tümörün büyümesi, gelişmesi ve metastaz gibi durumlarda  önemli rol oynayan bir serbest radikal olduğu ileri sürülmektedir.  Nitrik oksit sentaz (NOS) enzim ailesi L-sitrulinin, L-arginine dönüşümü sırasında Nitrik oksit (NO),  sentezine aracılık eden bir grup katalizörlerdir. Bu çalışmanın amacı Nitrik Oksit Sentaz (NOS3) Glu298Asp polimorfizmini meme kanserli hastalarda araştırmaktır. Bu amaçla 49 meme ve 89 sağlıklı kontrol grubu çalışmaya dahil edilmiştir. Çalışma gruplarımızda NOS3 Glu298Asp polimorfizmi periferal kan örnekleri kullanarak PCR-RFLP yöntemiyle tayin edilmiştir. Meme kanserli hastalarda NOS3 Glu298Asp genotip dağılımı [GG: 2 (4.1%), GT: 22(44.9%), TT: 25(51%)] sağlıklı kontrol grubundan [GG: 25(28.1%), GT: 34(38.2%), TT: 30(33.7%)] istatistiksel olarak anlamlı derecede farklı bulunmuştur (p=0.024). NOS3 Glu298Asp gen varyasyonu T allel frekansı meme kanserli hastalarda kontrol grubuna göre  anlamlı şekilde yüksek olarak saptanmıştır (χ2=7.229; p=0.007; OR: 1.228;%95 CI: 1.065-1.415).

Çalışmamız sonucunda NOS3 Glu298Asp polimorfizminin meme kanseri riski ile ilişkili olabileceği kanısına varılmıştır.


Anahtar kelimeler


oksidatif stres, NOS3 geni, varyasyon

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