Mustafa Gündoğar, Güzide Pelin Sezgin, Erhan Erkan, Özgün Yusuf Özyılmaz


Purpose: The aim of this study was to evaluate the effect of a commercially available irrigant, the Qmix, on the push out bond strength of Endosequence BC which is a bioceramic based root canal sealer.

Material and Methods: Sixty extracted maxillary central incisors were prepared with Protaper Next rotary instrument up to X4 (# 40). The specimens were randomly divided into four groups of equal sample size (n=15), according to the final irrigation regimen as follows: Group 1: 2.5% NaOCl (control), Group 2: 17% EDTA, Group 3: 2% CHX, Group 4: QMix 2 in 1. After rinsing, teeth were obturated using gutta-percha with Endosequence BC sealer. Slices were obtained from coronal, middle, and an apical section of the root canal. Push-out test was performed to measure the bond strength between the root canal dentin and the sealer. Data was statistically analyzed.

Results: The push-out bond strength of the root canal sealer was significantly affected by the type of irrigation solution. Highest bond strengths were found in canals irrigated with Qmix solution, and these results were regardless of anatomical section of the root canal (p< 0.05).

Conclusion: Final irrigation of the root canals with QMix had a positive effect on the bond strength of Endosequence BC sealer throughout the root canal.




Endosequence BC sealer; irrigant; push-out test; Qmix; endodontics



Hulsmann M, Stryga F. Comparison of root canal preparation using different automated devices and hand instrumentation. J Endod 1993;19(3):141-145.

Peters OA, Laib A, Gohring TN, Barbakow F. Changes in root canal geometry after preparation assessed by high-resolution computed tomography. J Endod 2001;27(1):1-6.

Peters OA, Peters CI, Schonenberger K, Barbakow F. Protaper rotary root canal preparation: Assessment of torque and force in relation to canal anatomy. Int Endod J 2003;36(2):93-99.

Rodig T, Hulsmann M, Muhge M, Schafers F. Quality of preparation of oval distal root canals in mandibular molars using nickel-titanium instruments. Int Endod J 2002;35(11):919-928.

Wu MK, Wesselink PR. A primary observation on the preparation and obturation of oval canals. Int Endod J 2001;34(2):137-141.

Naenni N, Thoma K, Zehnder M. Soft tissue dissolution capacity of currently used and potential endodontic irrigants. J Endod 2004;30(11):785-787.

Violich DR, Chandler NP. The smear layer in endodontics - a review. Int Endod J 2010;43(1):2-15.

Marending M, Paque F, Fischer J, Zehnder M. Impact of irrigant sequence on mechanical properties of human root dentin. J Endod 2007;33(11):1325-1328.

Zehnder M, Schmidlin P, Sener B, Waltimo T. Chelation in root canal therapy reconsidered. J Endod 2005;31(11):817-820.

Baumgartner JC, Mader CL. A scanning electron microscopic evaluation of four root canal irrigation regimens. J Endod 1987;13(4):147-157.

Ma J, Wang Z, Shen Y, Haapasalo M. A new noninvasive model to study the effectiveness of dentin disinfection by using confocal laser scanning microscopy. J Endod


Elnaghy AM. Effect of qmix irrigant on bond strength of glass fibre posts to root dentine. Int Endod J 2014;47(3):280-289.

Eliot C, Hatton JF, Stewart GP, Hildebolt CF, Jane Gillespie M, Gutmann JL. The effect of the irrigant qmix on removal of canal wall smear layer: An ex vivo study. Odontology 2014;102(2):232-240.

Bui TB, Baumgartner JC, Mitchell JC. Evaluation of the interaction between sodium hypochlorite and chlorhexidine gluconate and its effect on root dentin. J Endod 2008;34(2):181-185.

Singh G, Gupta I, Elshamy FM, Boreak N, Homeida HE. In vitro comparison of antibacterial properties of bioceramic-based sealer, resin-based sealer and zinc oxide eugenol based sealer and two mineral trioxide aggregates. Eur J Dent 2016;10(3):366-369.

Kokkas AB, Boutsioukis A, Vassiliadis LP, Stavrianos CK. The influence of the smear layer on dentinal tubule penetration depth by three different root canal sealers: An in vitro study. J Endod 2004;30(2):100-102.

Ballal NV, Moorkoth S, Mala K, Bhat KS, Hussen SS, Pathak S. Evaluation of chemical interactions of maleic acid with sodium hypochlorite and chlorhexidine gluconate. J Endod 2011;37(10):1402-1405.

Kara Tuncer A. Effect of qmix 2in1 on sealer penetration into the dentinal tubules. J Endod 2015;41(2):257-260.

Razmi H, Bolhari B, Karamzadeh Dashti N, Fazlyab M. The effect of canal dryness on bond strength of bioceramic and epoxy-resin sealers after irrigation with sodium hypochlorite or chlorhexidine. Iran Endod J 2016;11(2):129-133.

Attal JP, Asmussen E, Degrange M. Effects of surface treatment on the free surface energy of dentin. Dent Mater 1994;10(4):259-264.

Dogan Buzoglu H, Calt S, Gumusderelioglu M. Evaluation of the surface free energy on root canal dentine walls treated with chelating agents and naocl. Int Endod J 2007;40(1):18-24.

Erickson RL. Surface interactions of dentin adhesive materials. Oper Dent 1992;Suppl 5:81-94.

Hu X, Ling J, Gao Y. Effects of irrigation solutions on dentin wettability and roughness. J Endod 2010;36(6):1064-1067.

Ballal NV, Tweeny A, Khechen K, Prabhu KN, Satyanarayan, Tay FR. Wettability of root canal sealers on intraradicular dentine treated with different irrigating solutions. J Dent 2013;41(6):556-560.

Kuruvilla JR, Kamath MP. Antimicrobial activity of 2.5% sodium hypochlorite and 0.2% chlorhexidine gluconate separately and combined, as endodontic irrigants. J Endod 1998;24(7):472-476.

Akisue E, Tomita VS, Gavini G, Poli de Figueiredo JA. Effect of the combination of sodium hypochlorite and chlorhexidine on dentinal permeability and scanning electron microscopy precipitate observation. J Endod 2010;36(5):847-850.

Aranda-Garcia AJ, Kuga MC, Vitorino KR, Chavez-Andrade GM, Duarte MA, Bonetti-Filho I, Faria G, So MV. Effect of the root canal final rinse protocols on the debris and smear layer removal and on the push-out strength of an epoxy-based sealer. Microsc Res Tech 2013;76(5):533-537.

Dai L, Khechen K, Khan S, Gillen B, Loushine BA, Wimmer CE, Gutmann JL, Pashley D, Tay FR. The effect of qmix, an experimental antibacterial root canal irrigant, on removal of canal wall smear layer and debris. J Endod 2011;37(1):80-84.

Stojicic S, Shen Y, Qian W, Johnson B, Haapasalo M. Antibacterial and smear layer removal ability of a novel irrigant, qmix. Int Endod J 2012;45(4):363-371.

Shokouhinejad N, Hoseini A, Gorjestani H, Shamshiri AR. The effect of different irrigation protocols for smear layer removal on bond strength of a new bioceramic sealer. Iran Endod J 2013;8(1):10-13.

Dogan H, Qalt S. Effects of chelating agents and sodium hypochlorite on mineral content of root dentin. J Endod 2001;27(9):578-580.

Uzunoglu E, Turker SA, Karahan S. The effect of increased temperatures of qmix and edta on the push-out bond strength of an epoxy-resin based sealer. J Clin Diagn Res 2015;9(7):ZC98-ZC101.

Carvalho NK, Prado MC, Senna PM, Neves AA, Souza EM, Fidel SR, Sassone LM, Silva EJ. Do smear-layer removal agents affect the push-out bond strength of calcium-silicate based endodontic sealers? Int Endod J 2016, 10.1111/iej.12662

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