KI-67 AND CYCLOOXYGENASE-2 EXPRESSIONS IN ODONTOGENIC KERATOCYSTS, DENTAL FOLLICLE AND AMELOBLASTOMA-AN IMMUNOHISTOCHEMICAL STUDY-Odontojen Keratokistler, Dental Foliküller ve Ameloblastomalarda Cyclooxygenase-2 ve Ki67’nin İmmunohistokimyasal Yöntemle Ar

Çiğdem ÖZÇAMUR-GÜNEŞ, Vakur OLGAÇ, Merva SOLUK-TEKKEŞIN, Banu GÜRKAN KÖSEOĞLU
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Abstract


Odontojen Keratokistler, Dental Foliküller ve Ameloblastomalarda Cyclooxygenase-2 ve Ki67’nin İmmunohistokimyasal Yöntemle Araştırılması

ABSTRACT
Purpose: Ki-67 and COX-2 are two markers which are commonly used to assess tumor proliferation. The aims of this study were to evaluate and compare the presence of Ki-67 and COX-2 in odontogenic keratocyst (OKC), radicular cyst (RC), ameloblastoma (AML) and dental follicle (DF) in order to determine proliferative potentials of such lesions. 

Materials and Methods: This study has been conducted on 80 samples obtained from non-smoking, healthy subjects diagnosed as having either OKC, RC, AML or DF. 20 samples obtained from healthy mucosa served as controls. Slides prepared from paraffin-embedded sections were immunohistochemically stained for Ki-67 and COX-2 expressions.

Results: Ki-67 and COX-2 expressions in the OKC and AML groups were found to be significantly higher than those observed in the RC, DF and healthy mucosa groups (p < 0.005 for each).

Conclusion: High levels of Ki-67 and COX-2 staining in OKC and AML can be considered as the indicators of high proliferation tendency and mitotic activity. These markers could provide valuable tools for the treatment process when they are available for widespread use to detect cases that have significant relapse potential and aggressive behavior.

Keywords: Odontogenic cysts, ameloblastoma, dental follicle, Ki-67, COX-2

ÖZ
Amaç: Ki-67 ve COX-2, tümör proliferasyonunu değerlendirmek için yaygın olarak kullanılan iki belirteçtir. Bu çalışmanın amacı radiküler kist, keratokist, ameloblastoma ve tam gömük yirmi yaş dişlerinin dental folikülündeki Ki-67 ve COX-2 expresyonlarını değerlendirmek ve sağlıklı dokularla karşılaştırmaktır.

Gereç ve Yöntem: Bu çalışma sistemik hastalığı bulunmayan, sigara ve ilaç kullanmayan 80 hastadan elde edilen odontojenik keratokist, ameloblastoma, radikuler kist ve dental folikul örnekleri üzerinde yapılmıştır. Sağlıklı mukozalardan alınan 20 adet örnek kontrol grubu olarak değerlendirilmiştir.
Hazırlanan kesitlerde Ki-67 ve COX-2 expresyonu immunohistokimyasal yöntemlerle boyanarak araştırılmıştır.

Bulgular: Odontojenik keratokist ve ameloblastoma gruplarındaki Ki-67 ve COX-2 ekspresyonları radiküler kist, dental folikül ve sağlıklı mukoza gruplarına göre anlamlı derecede yüksek bulunmuştur (her biri için p < 0.005).

Sonuç: Keratokist ve ameloblastomada yüksek bulunan Ki-67 ve COX-2 değerleri bu lezyonların proliferasyon eğilimlerinin ve belirgin mitotik aktivitelerinin göstergesi olarak kabul edilebilirler. Bu
belirteçler, yüksek nüks eğilimi olan ve agresif özellik gösterebilecek olguların saptanmasında yaygın olarak kullanıldığında tedavi sürecine belirgin katkıları olacaktır.

Anahtar kelimeler: Odontojenik kistler, ameloblastoma, dental folikül, Ki-67, COX-2


Keywords


Odontogenic cysts, ameloblastoma, dental follicle, Ki-67, COX-2

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DOI: http://dx.doi.org/10.17096/jiufd.12386

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