INTERFERON- GAMMA LEVELS IN THE ODONTOGENIC CYST FLUIDS REGARDING BACTERIUM CONTENT-Odontojenik Kist Sıvılarının Bakteri İçeriğine Göre İnterferon-gamma Düzeyleri

Suzan ÇINAR, Sevgi ÇİFTÇİ, Fahriye KESKİN, Sırmahan ÇAKARER, Fırat SELVİ, Taylan CAN, Cengizhan KESKİN, Günnur DENİZ
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Abstract


ABSTRACT

Purpose: Odontogenic cysts (OCs) are pathological lesions that include liquid or semi-liquid surrounded with epithelium. Radicular cysts’ (RCs) and odontogenic keratocysts’ (OKCs) are common odontogenic cysts of jaws, and may reach to a substantial size without symptoms for a long time. It is known that cytokines secreted during infection and inflammation regulate the immune response. This study aims to describe the relationship between bacteria as infection agents and the levels of interferon-gamma (IFN-γ) cytokine of innate and adaptive immune response.

Materials and Methods: OC fluid samples with a history of infection were collected from a total of 39 OCs consisting 25 samples of odontogenic keratocysts (OKC) and 14 samples of radicular cysts (RC). Anaerobic bacteria detection was performed by a polymerase chain reaction (PCR) based on bacterial 16S rRNA genes. IFN-γ levels in OC fluids were determined using the luminex method.

Results: No significant differences in IFN-γ levels and T cell type 1 cytokine responses were observed between the cystic fluid samples classified on the basis of age, gender, cyst-type, cyst-size, bacterial species and the number of bacterial species contained. The measured concentrations IFN-γ, which is a helper T cell type 1 cytokine were consistent with published data from experimental animal models, immunohistochemical studies, and molecular studies.

Conclusion: Luminex method can detect the concentration of many different types of protein in a small sample volume and is suitable for determining the protein content of odontogenic cysts.

Keywords: Odontogenic keratocyst, radicular cysts, interferon-gamma, bacterial species, polymerase chain reaction (PCR)

ÖZ

Amaç: Odontojenik kistler (OK), etrafı epitel ile çevrili içinde sıvı veya yarı-sıvı bulunan patolojik oluşumlardır. Odontojenik keratokistler (OKC) ve radiküler kistler (RC) uzun zaman belirti vermeyerek belirli bir büyüklüğe ulaşabilen, ağızın yaygın odontojenik kistleridir. Enfeksiyon ve inflamasyon sırasında salgılanan sitokinlerin bağışıklık yanıtını düzenlediği konusunda da bilgiler mevcuttur. Bu çalışma, enfeksiyon ajanları bakteriler ile doğal ve kazanılmış immün yanıt sitokinlerinden interferon-gamma (IFN-γ) düeyi ilişisinin tanılanmasıamacıla düenlenmişir.

Gereç ve Yöntem: Yirmibeş odontojenik keratokist (OKK) ve 14 radiküler kist (RK) olmak üzere enfeksiyon geçmişine sahip toplam 39 OK sıvı örneği alınmıştır. Anaerobik bakterilerin saptanması amacıyla, bakteriyel 16S RNA gen temelli polimeraz zincir reaksiyonu (PZR) kullanılmıştır. OK sıvısındaki IFN-γ düeyi luminex yötemiyle belirlenmişir.

Bulgular: Yaşa, cinsiyete, kist tipine, büyüklüğüne, içerdiği bakteri türü ve tür sayısına göre gruplandırılan kist sıvılarının IFN-γ düeylerinde anlamlıfark ve buna bağıolarak T hüre tip 1 sitokin yanıısaptanmamışı. YardıcıT hüre tip 1 sitokinlerinden olan IFN-γ düeyleri daha öce yayılanan hayvan deneyleri, immüohistokimya ve moleküer çlışalarla uyumlu bulunmuşur.

Sonuç: Az miktardaki örnekte pek çok proteinin düzeyini belirleyen lumineks metodu odontojenik kist sıvılarının protein içeriğini belirlemek için uygundur.

Anahtar kelimeler: Odontojenik keratokist, radiküler kist, interferon-gama, bakteri türleri, polimeraz zincir reaksiyonu (PZR)

 


Keywords


Odontogenic keratocyst, radicular cysts, interferon-gamma, bacterial species, polymerase chain reaction (PCR)

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DOI: http://dx.doi.org/10.17096/jiufd.38727

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Corresponding Author: Suzan ÇINAR Department of Immunology Institute of Experimental Medicine (DETAE) Istanbul University,Çapa/Fatih-Istanbul Phone: 0212 414 2000-33342 e-mail: scinar@istanbul.edu.tr




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